| Vector |
Construct size (kb): 45.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 37.0
Cross references: DNA Seq. Acc.: L01962
DNA Seq. Acc.: L01963
DNA Seq. Acc.: L01964
DNA Seq. Acc.: L01965
DNA Seq. Acc.: L01966
DNA Seq. Acc.: L01967
DNA Seq. Acc.: L01968
DNA Seq. Acc.: L01969
DNA Seq. Acc.: L01970
DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983 Insert lengths(kb): 37.0 Tissue: lymphoblast Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide [SCN4A] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--12.5, 10.5, 5.3, 5.0, 3.8 (doublet), 3.6, 0.8; BglII--15.5 (doublet), 12.0; EcoRI--20.0, 12.0, 8.8, 2.1; HindIII--14.5, 12.0, 7.1, 3.75, 3.6, 2.1; SstI--13.5, 9.4, 5.6, 5.3, 3.4, 1.75, 1.35, 1.05, 0.86. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Extends from exon 1 into exon 24, from the 5' untranslated region into the 3' untranslated region. |
