| 產品名稱 |
pVL-RabGGTalpha |
| 商品貨號 |
B205520 |
| Designations |
pVL-RabGGTalpha |
| GenBank Number |
L10415
|
| Species |
Rattus norvegicus, rat |
| Depositors |
MC Seabra, S Armstrong |
| Applications |
in suitable host, produces protein Rab geranylgeranyl transferase II, alpha subunit |
| Vector |
Construct size (kb): 12.19999980926514 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.599999904632568 Tissue: brain Gene product: Rab geranylgeranyl transferase II, alpha subunit [GNGNT2A*] Target Gene: Rab geranylgeranyl transferase II, alpha subunit |
| Insert Size (kb) |
2.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 2.6; SmaI--11.8, 0.5; XhoI--9.6, 2.6. The insert contains the following restriction sites (approximate kb from the 5' end): ScaI--0.26, 1.27; SmaI--0.43; XhoI--0.46; StyI--1.75. When pVL-RabGGTalpha and pVL-RabGGTbeta (ATCC 87154, 87155) are used to generate recombinant baculoviruses and infect Sf9 cells, the active dimer is produced. |
| References |
Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464
Luckow VA, Summers MD. Signals Important for high-level expression of foreign genes in Autographa californica Nuclear Polyhedrosis Virus expression vectors. Virology 167: 56-71, 1988. PubMed: 3142147
|
