| 產品名稱 | TeloHAEC-GFP |
|---|---|
| 商品貨號 | B204361 |
| Organism | Homo sapiens, human |
| Tissue | aorta |
| Cell Type | endothelial |
| Product Format | frozen |
| Morphology | endothelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells containing SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 34 years old |
| Gender | female |
| Applications | TeloHAEC-GFP (ATCC CRL-4054) cells retain important endothelial cell characteristics such as CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation. When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages |
| Images | 
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| Derivation | TeloHAEC-GFP is a clonal cell line by stably expressing EmGFP under EF1α promoter in TeloHAEC (ATCC CRL-4052) cell line. |
| Clinical Data | female
34 |
| Antigen Expression | Positive for CD31/PECAM-1 expression and capable of uptaking Low Density Lipoprotein (LDL). |
| Complete Growth Medium | The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041). Optional: Add 0.3ug/mL Puromycin (Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent.
|
| Cryopreservation | 90% FBS, 10% DMSO |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | D13S317: 9, 12 D7S820: 10, 11 D16S539: 12, 13 vWA: 15, 16 Amelogenin: X TPOX: 8 CSF1PO: 11, 12 TH01: 6, 8 |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | ATCC |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
