| 產(chǎn)品名稱 |
NCI-H748 [H748] |
| 商品貨號 |
B204037 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; Derived from metastatic site: lymph node |
| Product Format |
flask |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E, carcinoma; classic small cell lung cancer |
| Age |
62 years |
| Gender |
male |
| Ethnicity |
Black |
| Karyotype |
t(1;3)del(3)(p14-p23) |
| Derivation |
The line was established in July 1984. |
| Clinical Data |
male
Black
62 years |
| Comments |
The line was established in July 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 12 D5S818: 10 D7S820: 10,11 THO1: 7,9 TPOX: 10,12 vWA: 17,19 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1984 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
