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Size (kb): 5.0000000000000000

Vector: pAKA19 (plasmid)

Construction: pAKA16, pAKA18

Marker(s):ampR

Construct size (kb): 5.0

Features: insert detection: lacZ'
marker(s): ampR
other: mob
replicon: pMB1
MCS: HindIII/SphI/PstI/.../NruI/HindIII

integrating vector

suicide vector

vector permitting visual detection of recombinants

vector useful for transposon mutagenesis

Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; BamHI--5.0; NdeI--5.0.

A suicide vector for allelic exchange or transposon mutagenesis in Pasteurella haemolytica or P. multocida.

Constructed by blunt-end ligating a 485 bp HaeII fragment (from pAKA16, trimmed with T4 DNA polymerase, and containing lacZ' and an MCS) to ScaI-digested pAKA18 (containing the pMB1 replicon).

Temperature: 37.0°C