| 產品名稱 |
pFL61 |
| 商品貨號 |
B202854 |
| Designations |
pFL61 |
| Depositors |
F Lacroute |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information |
Size (kb): 5.425 DESCRIPTION OF VECTOR:
Intact vector size: 5.425
Type of vector: plasmid
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron Vector: pFL61 (plasmid) Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.425 Features: marker(s): ampR, URA3
replicon: pMB1, 2 micron |
| Applications |
YE-type (episomal) shuttle vector expression vector shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; BglII--4.3, 1.1; ClaI--4.9, 0.5. A YE-type shuttle expression vector. The order of the major features in this plasmid is: EcoRI/MCS/XbaI - pGK5' - NotI - BstXI - BstXI - NotI - pGK3' - URA3 - 2 micron - pMB1 ori - AmpR. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803
Francois Lacroute, personal communication
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
