Digestion with HindIII gives the following sizes (kb): 3.5, 3.0, 1.9, 1.4(doublet), small fragment. If any telomere DNA has been deleted, the doublet will be resolved and a new band will appear. Restriction digests of the clone give the following sizes (kb): HindIII--3.7, 3.0, 1.9, 1.47; SalI--12.0. An occasional isolate will spontaneously delete small amounts of telomere sequence. Check by digesting the plasmid with HindIII. Due to ligation compatibility with sites in the polylinker, DNA fragments from BssHII, EagI, NaeI, NarI, NruI, SmaI and XhoI digests may also be cloned in this vector. A yeast artificial chromosome vector for genomic library construction using DNA digested with infrequent restriction enzymes. Constructed by removing the ClaI and SalI sites from pYAC3 and inserting a polylinker in the SnaBI site within SUP4. SUP4 still functions. |
