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pMF-alpha8 [pMFalpha8]
pMF-alpha8 [pMFalpha8]
規格:
貨期:
編號:B202315
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pMF-alpha8 [pMFalpha8]
商品貨號 B202315
Designations pMF-alpha8 [pMFalpha8]
Depositors A Miyajima
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pMF-alpha8 (plasmid)
Promoters: Promoter alpha factor 1
Construction: pTRP584, pMFalpha5
Marker(s):TRP1,ampR
Construct size (kb): 7.0
Features: marker(s): ampR, TRP1
promoter: alpha factor 1
replicon: pMB1, 2 micron, ARS1
terminator: TRP5
Applications
YX-type (expression) shuttle vector
expression vector
shuttle vector
vector with sequences for protein export
Comments
Shuttle expression vector permitting processing and secretion of eukaryotic proteins. Cloning sites are immediately downstream of the first processing site for the alpha factor leader.
Constructed by inserting a modified alpha promoter leader sequence into pTRP584, replacing the StuI site in the TRP1-ARS1 fragment with a PvuII site, so that the StuI site in the alpha factor processing region is unique.
The order of the major features in this plasmid is: 2 micron ori - alpha factor leader - StuI - XhoI - TRP5 - EcoRI - pMB1 ori - ampR - EcoRI - ARS1 - PvuII - TRP1 - EcoRI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Miyajima A, et al. Secretion of mature mouse interleukin-2 by Saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone alpha-factor. Gene 37: 155-161, 1985. PubMed: 3902570

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