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Construct size (kb): 4.300000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 12
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: T25343; EST: EST017; Primer: T7
DNA Seq. Acc.: T25344; EST: EST017R; Primer: T3; THC id:
THC98013

Insert lengths(kb): 1.399999976158142

Tissue: BL29 cell line Burkitt's lymphoma

Disease: Burkitt's lymphoma

Gene product: DNA Segment, single copy, expressed

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; BamHI--4.3; HindIII--4.3; KpnI--4.3; PstI--3.1, 1.2.

Weak signals are also detected on chromosomes 17 and X.