Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
monkey, Macaca nemestrina, West Malaysia, 1969
Product Format
frozen
Type Strain
yes
Comments
Cloned strain.
Variation in kinetoplast minicircle and maxicircle DNA sequences
Medium
ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor
D Weinman
Year of Origin
1969
References
Weinman D. Trypanosoma cyclops n. sp.: a pigmented trypanosome from the Malaysian primates Macaca nemestrina and M. ira. Trans. R. Soc. Trop. Med. Hyg. 66: 628-636, 1972. PubMed: 4627177
Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003
