An expression vector containing a synthetic consensus Escherichia coli RNA polymerase promoter, tandem synthetic lactose operators, a HindIII CAT gene cartridge as well as the pBR327 amp and tet resistance genes.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698
