| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 8.595, 0.427 (vector); 5.15, 4.26, 3.364, 2.932, 2.521, 1.903, 1.786, 1.687, 1.62, 1.281, 1.225, 1.011, 0.965, 0.936, 0.933, 0.904, 0.831, 0.780, 0.763, 0.700, 0.649. 0.471. 0.427. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 168828-209847 of the sequence of chromosome VIII. |
| References |
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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