| 產品名稱 |
Choanoeca perplexa Ellis |
| 商品貨號 |
B200575 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Atlantic Ocean off Plymouth, England, 1977 |
| Product Format |
frozen |
| Type Strain |
no |
| Comments |
Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 |
| Medium |
ATCC® Medium 1361: Marine flagellate medium
|
| Growth Conditions |
Temperature: 15.0°C Duration: grown with mixed bacteria Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask. |
| Subcultivation |
Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask. |
| Name of Depositor |
BS Leadbeater |
| Year of Origin |
1977 |
| References |
Leadbeater BS. . J. Mar. Biol. Assoc. U.K. 57: 285-301, 1977.
Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774
|
