| Comments |
A recombinant virus containing the HSV-1 Bgl/BamH1 TK-fragment in the vaccinia VTK-79L vector. This clone shows a higher level of TK expression than variants vP-2, vP-4 (ATCC VR-2033), or vP-6 (ATCC VR-2029). A BglII-BamHI fragment encoding HSV-1 TK (minus the regulatory sequences) was cloned into one of the BamHI sites of an AvaI fragment from the vaccinia L-variant. This construct was then cloned into VTK-79L (ATCC VR-2056) by in vivo recombination. |
| References |
Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986
Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988
Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520
|
