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Size (kb): 2.8499999046325680

Vector: pTM-N (plasmid)

Promoters: Promoter for expression T7 (phi10)

Construction: pET-3a,pIN-III-ompA3

Marker(s):ampR

Construct size (kb): 2.849999904632568

Features: marker(s): ampR
other: sequence for 3' mRNA secondary structure
other: sequence for 5' mRNA secondary structure
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NcoI...BamHI
ribosome-binding site: ompA Shine-Dalgarno
signal peptide coding region: ompA

expression vector

vector with sequences for protein export

Restriction digests of the clone give the following sizes (kb): BamHI--2.9; EcoRI--2.9; NcoI--2.9.

Expression vector permitting overproduction and secretion of foreign proteins in an unfused state. The ompA signal sequence directs proteins to the periplasmic space.

pTM-N differs from pTO-N (GenBank accession M34008) only in the bla (ampR) gene. The PstI site in M34008 has been removed in pTM-N.

Temperature: 37.0°C