-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
HCC1954 BL is a B lymphoblastoid cell line initiated from peripheral blood lymphocytes from the same patient as HCC1954 (ductal carcinoma of the breast) by transformation with Epstein-Barr virus (EBV). The cell line was derived from a 61 year-old East Indian female with stage IIA, grade 3, primary ductal carcinoma of the breast.? DNA isolated from HCC1954 is available as ATCC CRL-2338-D.
Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio 3 1.8
· Tested and verified for PCR amplification and restriction digestion.
