人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CCD-1141Sk
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
CCD-1141Sk
CCD-1141Sk
規格:
貨期:
編號:B199533
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 CCD-1141Sk
商品貨號 B199533
Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 44 years
Gender male
Ethnicity Caucasian
Applications
The line was established from normal skin taken from the abdomen.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The line was established from normal skin taken from the abdomen.
Clinical Data
male
Caucasian
44 years
Comments
The line was established from normal skin taken from the abdomen.
Cells senesce after approximately 50 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor F.Haq, ATCC
Year of Origin August, 2003
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 扒开女人内裤猛进猛出免费视频| 亚洲五月综合缴情在线观看| 一本一久本久a久久精品综合| 国产亚洲精品自在久久| 久久中文字幕人妻熟av女蜜柚m| 欧美午夜理伦三级在线观看| 99日本精品永久免费久久| 成人网站国产在线视频内射视频| 国产露脸150部国语对白| 国产欧美日韩| 日韩a毛片免费观看| 国产产品亚洲产品| 精品国产制服丝袜高跟| 男女啪啪做爰高潮免费网站| 国产午夜福利在线播放87 | 日本免费一区二区三区| 久久久久久综合网天天| 国产农村乱人伦精品视频| 韩国精品久久久久久无码| 无码少妇一区二区性色av| 欧美成人aaa片一区国产精品| 美女被张开双腿日出白浆| 免费国产污网站在线观看| 丝袜 中出 制服 人妻 美腿| 最近免费中文字幕大全免费版视频 | 亚洲中文字幕aⅴ天堂| 国产精品后入内射日本在线观看 | 天堂在/线中文在线资源 官网| 漂亮人妻被强中文字幕久久 | 精品黑人一区二区三区| 国产成人精品三级在线影院| 色欲久久久中文字幕综合网| 污污内射在线观看一区二区少妇 | 任你躁在线精品免费| 无码人妻啪啪一区二区| 亚洲人成电影网站色迅雷| 奇米影视7777狠狠狠狠影视| 国产又色又爽又黄刺激在线视频| 国产98色在线 | 国| 国自产拍偷拍精品啪啪av| 亚洲欧美成aⅴ人在线观看|