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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 6.3590002059936520

DESCRIPTION OF VECTOR:
Intact vector size: 6.359
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: TEF, <-, 2339-2741
marker(s): ampR, <-, 3859-4789
replicon: 2 micron, ->, 4889-6229

Vector: p426 TEF (plasmid)

Promoters: Promoter for expression TEF

Construction: pRS426 (ATCC 77107)

Marker(s):URA3,ampR

Construct size (kb): 6.359000205993652

Features: marker(s): URA3
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

Restriction digests of the clone give the following sizes (kb): BamHI--6.5; SacI/XBaI--3.2, 2.7, 0.44; XhoI--6.5.

High copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669