| 產品名稱 |
DT301 |
| 商品貨號 |
B198865 |
| Designations |
DT301 |
| Species |
Corynebacterium diphtheriae (Kruse) Lehmann and Neumann |
| Depositors |
President and Fellows of Harvard College, JR Murphy, President and Fellows of Harvard College |
| Vector |
Construct size (kb): 0.0 Insert ends: Sau3A1 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Corynebacterium diphtheriae
Gene name: diphtheria toxin, fragment B
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb):
Cross references: Gene product: diphtheria toxin, fragment B |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2. Constructed by cloning a Sau3A1 fragment from the tox gene including all of the fragment B gene. Fragment cloned is depicted as fragment B' in Fig. 3 of US Patent 4,675,382 dated June 23, 1987. Constructed from pUC8 and a small segment of the Corynebacteriophage beta tox-197 genome. |
| References |
Murphy JR. Hybrid protein. US Patent 4,675,382 dated Jun 23 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
