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Kluyveromyces lactis (Dombrowski) van der Walt
Kluyveromyces lactis (Dombrowski) van der Walt
規格:
貨期:
編號:B198105
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Kluyveromyces lactis (Dombrowski) van der Walt
商品貨號 B198105
Strain Designations [61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174]
Application
Degrades whey permeate
Hydrolyzes whey lactose
Produces L-methionine
Produces beta-galactosidase beta-D-galactosidase
Produces killer toxin
Produces L-methionine from whey permeate
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype a [k1+ k2+]
Preceptrol® no
Genome Sequenced Strain

Yes

Mating Type Mates with ATCC® 8651™
Ploidy haploid
Comments
Regulation of pyruvate dehydrogenase activity by E1alpha subunit levels
Resistant to killer toxin produced by Schwanniomyces occidentalis (ATCC® 44252™)
Carries two linear DNA plasmids, pGK11 and pGK12
Transcriptional regulation of beta-galactosidase gene
Effect of lactose permeabilization on beta-galactosidase production
Nonflocculating
Killer, immune
Genome sequencing strain (Genolevures Consortium, France).
Sequenced mitochondrial genome.
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

AAGGATCATTAAAGATTATGAATGAGTAGATTACTGGGGGAATCGCTGAATATGGCCTGCGCTTAATTGCGCGGCTAATTCTTGATTTTCTGCTATCAGTTTTCTTTCTCTCATCCTAAACACAATGGAGTTTTTTCTCTATGAACTACTTCCCTGGAGAGCTCGTCTCTCCAGTGGACATAAACACAAACAACATTTTGCATTATGAAAAACTATTTATCAAGAAATTTAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTTTTTCGGGTTAACTTGAAAGTGGCTAGCCGTTGCCTTCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAGGTTTGCGCCAATTCGTGGTAAGCTAGGGTCAATGAGCTTATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCTTAATCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAATACCCGCTGAACTTAAG


D1D2 region of the 26S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGAAGAAGGCAACTTTGTAGCTGGTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGATCCCAGTTATTTGTAAAGTGCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGGTTGGATAAATCCTCGGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACTGAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCG
Name of Depositor Lederle Labs.
Chain of Custody
ATCC <-- Lederle Labs. <-- N.C. Laffer 61, type C (Zygosaccharomyces lactis)
Isolation
Creamery, USA
Cross References

Nucleotide (GenBank) : KU729077 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729173 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : X72499 nucleotide sequence of KlGGS1 gene

Nucleotide (GenBank) : X81421 K.lactis gene for alpha-alpha-trehalose 1-D-glucohydrolase.

Nucleotide (GenBank) : CR382121 Kluyveromyces lactis strain NRRL Y-1140 chromosome A complete sequence.

References

Gunge N, et al. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J. Bacteriol. 145: 382-390, 1981. PubMed: 6257636

Wickerham LJ, Burton KA. Occurrence of yeast mating types in nature1,3. J. Bacteriol. 63: 449-451, 1952. PubMed: 14938317

Lacy LR, Dickson RC. Transcriptional regulation of the Kluyveromyces lactis beta- galactosidase gene. Mol. Cell. Biol. 1: 629-634, 1981. PubMed: 9279376

. . Biotechnol. Tech. 6: 289-292, 1992.

Schaffrath R, Meacock PA. Kluyveromyces lactis killer plasmid pGKL2: molecular analysis of an essential gene, ORF5. Yeast 11: 615-628, 1995. PubMed: 7483835

Bellal M, et al. Structural differences in parietal peptidomannans from flocculating strains of Kluyveromyces lactis. Can. J. Microbiol. 41: 323-335, 1995.

Luyten K, et al. Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake. Eur. J. Biochem. 217: 701-713, 1993. PubMed: 8223613

Dickson RC, Markin JS. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis. J. Bacteriol. 142: 777-785, 1980. PubMed: 6769910

Siso MI. Beta-galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem. 29: 565-568, 1994.

Bellal M, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 30: 641-648, 1995.

. . Enzyme Microb. Technol. 16: 303-310, 1994.

Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863

Zeeman AM, et al. Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 16: 611-620, 2000. PubMed: 10806423

Chen WB, et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000. PubMed: 11097913

Kitamoto HK, Nakahara T. Isolation of an L-methionine-enriched mutant of Kluyveromyces lactis grown on whey permeate. Process Biochem. 29: 127-131, 1994.

Dujon B, et al. Genome evolution in yeasts. Nature 430: 35-44, 2004.

Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000.

Bolotin-Fukuhara M, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis. FEBS Lett. 487: 66-70, 2000.

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Zivanovic Y, et al. Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces lactis. FEMS Yeast Res. 5: 315-322, 2005. PubMed: 15691736

梅經理 17280875617 1438578920
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周經理 17757487661 1296385441
于經理 18067160830 2088210172
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