Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0. Replication is temperature-sensitive. Can be used as a suicide vector for the delivery of transposable elements. Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5. |
