| 產品名稱 |
pBR:beta a |
| 商品貨號 |
B196976 |
| Designations |
pBR:beta a |
| Species |
Homo sapiens, human |
| Depositors |
Cetus Corp., A Belt, RK Saiki, Cetus Corp. |
| Vector |
Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR328
Intact vector size: 4.900
Type of vector: plasmid
Vector end: BamHI
Vector end: ?
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV BalI NcoI NruI PstI PvuI PvuII SalI SphI XmaIII
Polylinker sites:
Construction: pBR327 ori, pBR325 cml
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
promoter: none
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08858 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: HBB
Genomic copy number: unique
Gene name: hemoglobin, beta
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 p15.5
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 1.9
Cross references: Insert lengths(kb): 1.899999976158142 Gene product: hemoglobin, beta(globin, beta) [HBB] |
| Insert Size (kb) |
1.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 1.9; EcoRI--6.8; HindIII--6.8; BamHI/EcoRI--3.9, 2.2, 1.7. Includes first and second exons, first intron, and 5' flanking sequences. Isolated from pFC11. |
| References |
Saiki RK, Erlich HA. Method for detection of polymorphic restriction sites and nucleic acid sequences. US Patent 4,683,194 dated Jul 28 1987
Lawn RM, et al. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell 15: 1157-1174, 1978. PubMed: 728996
Collins FS, et al. G gamma beta+ hereditary persistence of fetal hemoglobin: cosmid cloning and identification of a specific mutation 5' to the G gamma gene. Proc. Natl. Acad. Sci. USA 81: 4894-4898, 1984. PubMed: 6205403
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
