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hGR(1-777)
hGR(1-777)
規(guī)格:
貨期:
編號(hào):B196156
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 hGR(1-777)
商品貨號(hào) B196156
Designations hGR(1-777)
Species Homo sapiens, human
Depositors MV Govindan
Applications
in another host, produces protein glucocorticoid receptor
Vector
Construct size (kb): 8.90000057220459
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKCR-2
Intact vector size: 5.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI, EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRL
Genomic copy number: unique
Gene name: glucocorticoid receptor
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: breast adenocarcinoma MCF7 cell line
Type of DNA: cDNA
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.88
Cross references:
Insert lengths(kb): 2.880000114440918
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: glucocorticoid receptor [GRL]
Target Gene: glucocorticoid receptor
Insert Size (kb) 2.880
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 3.1; EcoRI--5.8, 1.8, 0.86, 0.78; KpnI--9.4; XbaI--9.4; PstI--6.8, 2.2.
The orientation of the insert is: 5' BamHI, SmaI, KpnI, SacI, EcoRI, coding region for amino acids 1-777, XbaI/BamHI 3'.
Cross hybridizes with a 6.8 kb rat liver mRNA.
pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.
Constructed from overlapping cDNA clones.
References

LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621

Green S, Chambon P. Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor. Nature 325: 75-78, 1987. PubMed: 3025750

Govindan MV, et al. Cloning of the human glucocorticoid receptor cDNA. Nucleic Acids Res. 13: 8293-8304, 1985. PubMed: 2417195

Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476

Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951

Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537

Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589

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