| 產品名稱 |
45.1 DPbeta 003 |
| 商品貨號 |
B195260 |
| Designations |
45.1 DPbeta 003 |
| GenBank Number |
X03067
|
| Species |
Homo sapiens, human |
| Depositors |
EO Long |
| Vector |
Construct size (kb): 3.700000047683716 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.172000050544739 Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2) Gene product: major histocompatibility complex, class II, DP beta 2 (pseudogene) [HLA-DP2B] |
| Insert Size (kb) |
1.172 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.6, 1.1; SalI--3.4, 0.4; PvuII--3.4; XbaI--3.5; SmaI--3.7; PstI--2.4, 1.1, 0.4. The open reading frame contains 257 codons, flanked by 51 bp of 5' untranslated region and 259 bp of 3' untranslated region. Encodes complete sequence of DPw2 chain. BamHI site at 3' end is within SV40 sequence (coordinates 0.715-0.710). Insert cut out with BamHI will include approx. 35 bp of SV40 sequence. |
| References |
Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758
Dixon MJ, et al. Association of Treacher Collins syndrome and translocation 6p21.31/16p13.11: exclusion of the locus from these candidate regions. Am. J. Hum. Genet. 48: 274-280, 1991. PubMed: 1671319
|
