| 產品名稱 |
pBS-human p57 [KIP2] |
| 商品貨號 |
B195133 |
| Designations |
pBS-human p57 [KIP2] |
| GenBank Number |
U22398
|
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Vector |
Construct size (kb): 4.460000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1C
Other gene symbols: KIP2
Gene name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5
Tissue: embryo
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U22398
Nucleotides 1-1511 of the insert correspond to
nucleotides 1-1511 of U22398. Insert lengths(kb): 1.5 Tissue: embryo Gene product: cyclin-dependent kinase inhibitor 1C (p57, Kip2)(P57, kinase inhibitory protein 2 of cyclins (p57)) [KIP2] |
| Insert Size (kb) |
1.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--3.0, 1.6; EcoRI/HindIII--3.0, 1.55; EcoRI/PstI--3.0, 1.15, 0.47; PstI--3.5, 1.15; SmaI--3.2, 1.4. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.4, 0.49; HindIII--1.44; SmaI--0.25. |
| References |
Matsuoka S, et al. p57KIP2, a structurally distinct member of the p21CIP1 Cdk-inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. PubMed: 7729684
|
