| 產(chǎn)品名稱 | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
|---|---|
| 商品貨號(hào) | B194766 |
| Agent | Quantitative Synthetic Middle East respiratory syndrome coronavirus (MERS-CoV) RNA |
| Applications | ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications. |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen Specification range: 1 x 105 to 1 x 106 copies/μL 100 μL per vial with Biomatrica RNAstable |
| Storage Conditions | -70°C or colder |
| Intended Use | The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent. |
| Comments | Manufactured under ISO 13485 guidance Preparation includes fragments from the ORF1ab, ORF5, upper envelope (upE), ORF8b, nucleocapsid (N) protein gene, and 3' UTR regions The following primers and probe can be used with this nucleic acid preparation Ref   Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012.:Forward primer: GCAACGCGCGATTCAGTT Reverse primer: GCCTCTACACGGGACCCATA Probe: CTCTTCACATAATCGCCCCGAGCTCG |
| Name of Depositor | ATCC |
| Special Collection | RNA |
| References | Corman VM, et al. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill 17(49): pii=20334, 2012. Corman VM, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 17(39): pii=20285, 2012. Corman VM, et al. Performance and clinical validation of the RealStar? MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA. J. Clin. Virol. 60(2): 168-171, 2014. PubMed: 24726679 Lu X, et al. Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus. J. Clin. Microbiol. 52(1): 67-75, 2013. PubMed: 24153118 World Health Organization. Laboratory Testing for Middle East Respiratory Syndrome Coronavirus - Interim Guidance (Revised). WHO/MERS/LAB/15.1, June 2015. |
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