Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 0.75; BglI--1.75 (doublet), 1.7, 0.3 + smaller; EcoRI--4.0, 1.55; HindIII--4.7, 0,85; XbaI--5.6. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.65; BglI--1.22, 1.49; BglII--0.97; HindIII--0.71; NcoI--0.01; PvuII--1.52; SacI--0.69; SmaI--0.42; StuI--0.20, 0.43. Deposited with the understanding that the DNAs not be used in human experiments. Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence. |
