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pDP97
pDP97
規(guī)格:
貨期:
編號:B194451
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDP97
商品貨號 B194451
Designations pDP97
Species Homo sapiens, human
Depositors DC Page
Vector
Construct size (kb): 8.0
Insert
DNA: genomic
Insert lengths(kb): 5.300000190734863
Gene product: DNA Segment, repetitive [DYZ3]
Insert Size (kb) 5.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 2.7; HindIII--8.3; BamHI--8.3; PstI--4.5, 2.8, 0.57; AccI--5.0, 2.96.
Cross-hybridizes weakly to alphoid repeats on chromosomes 13, 14, and 15. Hybridize at high stringency (50 C, 50% formamide) to avoid cross-hybridization. Also a marker for deletion interval 4B.
Shows Y specificity, but at high stringency it detects a repeated Y specific EcoRI fragment of 5.5 kb (DXZ3).
References

Disteche CM, et al. Molecular detection of a translocation (Y;15) in a 45,X male. Hum. Genet. 74: 372-377, 1986. PubMed: 3793100

Page DC. Sex reversal: deletion mapping the male-determining function of the human Y chromosome. Cold Spring Harbor Symp. Quant. Biol. 51: 229-235, 1986. PubMed: 3472719

Wolfe J, et al. Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome. J. Mol. Biol. 182: 477-485, 1985. PubMed: 4040175

David C Page, personal communication

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