| 產品名稱 |
pRM189-1 [NEB816] |
| 商品貨號 |
B194028 |
| Designations |
pRM189-1 [NEB816] |
| Species |
Pantoea agglomerans (Beijerinck) Gavini et al. |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications |
produces protein modification methylase EagI produces protein restriction endonuclease NotI |
| Vector |
Construct size (kb): 9.399999618530273 |
| Insert |
DNA: genomic Insert lengths(kb): 5.199999809265137 Gene product: modification methylase EagI [hsdM] Target Gene: modification methylase EagI, restriction endonuclease NotI |
| Insert Size (kb) |
5.200 |
| Media |
ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of pRM189-1 give the following sizes (kb): NdeI/SalI--6.2, 1.7. Restriction digests of total plasmid DNA give the following sizes (kb): EcoRI/EcoRV--3.6, 1.7, 0.78; HindIII--9.4, 6.2; NdeI/SalI--9.4, 6.2, 1.7; PstI--9.4, 6.2; SalI--9.4, 6.2; AvaI--6.2, 0.9; EcoRV-- 6.0, 1.1. |
| References |
Morgan RD, et al. Isolated DNA encoding the NotI restriction endonuclease and related methods for producing the same. US Patent 5,371,006 dated Dec 6 1994
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
