Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 0.6; EcoRI--5.6; HindIII--5.6; HindIII/SalI--4.0, 1.6; SalI--5.6. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.4, 0.95; BglII--1.1; EcoRI--0.65; NcoI--0.2; PstI--0.3, 1.0; PvuII--0.3, 0.75. Deposited with the understanding that the DNAs not be used in human experiments. The protein produced is the constitutively activated R201C mutation (cysteine replacing arginine at codon 201). Encodes a hemagglutinin epitope replacing nt 475-489 of the GenBank sequence, followed by an altered serine codon (TCC) nt 490-492. Restriction site(s) at the 3' end of the insert for linearizing the construct: XhoI, NotI, HindIII. |
