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The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.

Myc/c-Myc, expressed

c-Myc plays a critical role in activating the transcription of growth-related genes. 

Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas.  

The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8 X 10³ to 1.5 X 10⁴ viable cells/cm² is recommended.
6. Incubate cultures at 37°C. Subculture when the cell concentration is between 1 X 10⁵ to 2 X 10⁵ cells/cm².

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Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814

Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037

Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823