| 產品名稱 |
Vaccinia virus ts mutant |
| 商品貨號 |
B193023 |
| Classification |
Poxviridae, Orthopoxvirus |
| Deposited As |
Vaccinia virus, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts11 |
| Applications |
The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM categories G and I, indicating that it is characterized by incomplete membranes with spicules, and produces some complete immature particles. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location F10, a viral serine/threonine kinase. |
| Biosafety Level |
2 CDC BMBL recommends vaccinationVaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This conditional lethal mutant, originally designated plaque isolate no.1327, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM categories G and I, indicating that it is characterized by incomplete membranes with spicules, and produces some complete immature particles. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location F10, a viral serine/threonine kinase. |
| Effect on Host |
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host: BSC-40 (ATCC CRL-2761)
Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions |
1-3 days at 31 C with 5% CO2 |
| Name of Depositor |
S Dales, R Condit |
| Special Collection |
NCRR Contract |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
|
