Restriction digests of the clone give the following sizes (kb): HindIII--5.6; HindIII/SmaI--3.0, 2.4; ScaI--3.3, 2.1. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.01, 0.46; NdeI--0.31, 1.51; ScaI--0.97; NcoI--1.83. Begins 21 nt upstream of the initiation codon and ends before an alternative spice site. Matches the sequence of a desmocollin published as desmocollin 4 (sequence accession D17427). |
