Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; HindIII--5.5; PstI--5.5. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions and facilitates the rescue in E. coli of plasmids where dimerization has occurred. The order of the major features in this plasmid is: ura4 - ars1 - SfiI/MCS/HindIII/lacZ' - pMB1 ori - ampR. |
