| 產品名稱 |
p47-8A |
| 商品貨號 |
B191727 |
| Designations |
p47-8A |
| GenBank Number |
M25665
|
| Species |
Homo sapiens, human |
| Depositors |
TL Leto |
| Applications |
produces protein neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) neutrophil cytosolic factor 1 |
| Vector |
Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NCF1
Genomic copy number: unique
Gene name: neutrophil cytosolic factor 1 (47kD, chronic granulomatous
disease, autosomal 1)
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q11.23
Tissue: HL-60 cell line (promyelocytic), differentiated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.34
Cross references: DNA Seq. Acc.: M25665 Insert lengths(kb): 1.3400000333786 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)(neutrophil cytosolic factor 1) [NCF1] Target Gene: neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) |
| Insert Size (kb) |
1.340 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BglII--4.1, 0.27; BamHI--3.7, 0.64; XhoI--3.5, 0.46, 0.36, 0.23; PstI--3.3, 0.6, 0.38, 0.26. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.15, 0.33; BamHI--0.62; XhoI--0.64, 0.75, 1.06; SmaI--1.05. Contains the complete coding sequence (nt 13-1185). Detects transcripts of 2.2 (faint) and 1.5 kb. |
| References |
Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022
Lomax KJ, et al. Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease. Science 245: 409-412, 1989. PubMed: 2547247
. . Science 246: 987, 1989.
|
