| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 9.2, 8.8, 7.8, 2.2, 1.1; EcoRI--16.0, 7.8, 4.7, 3.9, 2.7, 2.3, 2.0 (doublet), 1.1, 0.98, 0.58, 0.31; HindIII--11.0, 10.5, 9.8, 4.4, 3.5, 3.1, 2.1, 1.3; BglII--27.0, 7.8, 5.2, 4.2, 1.3, 0.53; SalI--17.0, 13.0, 7.0, 6.0, 2.5; NotI-->23.0, 8.6. Codominant segregation was observed in 30 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed after competitive hybridization with total human DNA. |
| References |
White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325
Bragg T, et al. Isolation and mapping of a polymorhphic DNA sequence (cTB14.16) on chromosome 10 (D10S33). Nucleic Acids Res. 16: 11391, 1988. PubMed: 2905055
Yusuke Nakamura, personal communication
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