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Construct size (kb): 3.450000047683716

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: VCL
Gene name: vinculin
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q22.1-q23; 10, 446.910000 cR
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.45
Cross references: DNA Seq. Acc.: M33308; EST: EST04831
Nucleotides 1-273 of the insert correspond to
nucleotides 4566-4294 of M33308 with 99% identity.
DNA Seq. Acc.: T06942; EST: EST04831; Primer: M13-21; THC
id: THC78924

Insert lengths(kb): 0.449999988079071

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: vinculin [VCL]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 0.45.

Based on the alignment with GenBank accession M33308 (5102 nt, CDS 51-3251) and the size of the insert, this clone appears to contain only 3' untranslated sequence.