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Distribution host: Escherichia coli JM109

Size (kb): 6.8000001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 6.800
Type of vector: plasmid
Cloning sites: PstI HincII ScaI SspI
Polylinker sites:
Construction: pDM2, pBGS8
Host range: Haemophilus influenzae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, spcR
replicon: pMB1, pRSF0885

Vector: pVT64 (plasmid)

Construction: pDM2, pBGS8

Marker(s):ampR,spcR

Construct size (kb): 6.800000190734863

Features: marker(s): ampR, spcR
replicon: pMB1, pRSF0885

shuttle vector

Restriction digests of the clone give the following sizes (kb): PstI--6.8; ScaI--6.8; SspI--6.8; BamHI--4.2, 2.4.

Cloning into the HincII, PstI, ScaI, or SspI sites inactivates ampR.

Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid.

Shuttle vector.

The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - spcR.

Temperature: 37.0°C