| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 5.8, 3.9, 2.1, 1.2, 0.9, 0.8; BamHI--17.0, 9.0; HindIII--8.0, 6.0, 3.6, 2.9, 1.7, 0.8; PstI--8.0, 7.8, 3.5, 2.4, 0.8; NotI--17.0, 9.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI. |
| References |
Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986
Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.96) on chromosome 16 [D16S157]. Nucleic Acids Res. 17: 9506, 1989. PubMed: 2573891
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