| 產品名稱 |
k2.8 |
| 商品貨號 |
B189957 |
| Designations |
k2.8 |
| GenBank Number |
L13974
|
| Species |
Homo sapiens, human |
| Depositors |
P Ney |
| Vector |
Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NFE2
Gene name: nuclear factor (erythroid-derived 2), 45kD
Contains complete coding sequence?: U
Chromosome: 12; Localization: 12 q13
Tissue: K562 cells
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.496
Cross references: DNA Seq. Acc.: L13974
Nucleotides 1-1496 of the insert correspond to
nucleotides 1-1496 of L13974. Insert lengths(kb): 1.496000051498413 Tissue: K562 cells Gene product: nuclear factor (erythroid-derived 2), 45kD(nuclear factor 45) [NFE2] |
| Insert Size (kb) |
1.496 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--2.7, 1.8; KpnI--3.3, 1.2; PstI--4.4; SalI--3.0, 1.3. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.10; BglI--0.21, 0.60, 0.65; KpnI--1.02; PstI--0.37; PvuII--0.30, 0.85, 1.13, 1.23; SacI--0.51; SmaI--0.06, 1.35; XbaI--0.20, 1.47. |
| References |
Ney PA, et al. Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner. Mol. Cell. Biol. 13: 5604-5612, 1993. PubMed: 8355703
|
