| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.517, 6.536, 0.538, 1.030, 1.374, 0.507, 2.434, 0.534, 6.513, 0.985, 0.730, 3.221, 0.658, 2.896, 3.649, 4.031. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.51, 0.65, 0.77, 0.99, 1.1, 1.4, 1.6, 2.5, 3.1, 3.4, 3.8, 4.1, 6.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70098, 70403, 70487, 70675, 70767, 70890, 70899, 70923. Corresponds to nt 506978-545587 of the sequence of chromosome VIII. |
| References |
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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