| 產品名稱 | NCI-H441 [H441] |
|---|---|
| 商品貨號 | B189813 |
| Organism | Homo sapiens, human |
| Tissue | lung |
| Cell Type | epithelial cell (KRAS CRM) |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | papillary adenocarcinoma |
| Gender | male |
| Applications | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.
The line has been used as a transfection host for expression of pulmonary surfactant protein (SP-B). |
| Intended Use | Certified Reference Material produced under an ISO 17034 accredited process. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 52; range = 44 to 59. This is a hyperdiploid human cell line. The modal chromosome number was 52, but cells with 53 chromosome counts also occurred at a high frequency. The rate of cells with higher ploidies was 4.9%. Over 14 marker chromosomes were common to all cells, and 6 or more others were present in some cells. Among the common markers were: der(1)t(1;1)(p36;p21), i(2q), der(11)t(11;12)(q21;q13), t(6p12q), der(9)t(9;?;14)(p24;?q11), t(12q22q) and 8 or more others. All these markers were present in a single copy per cell. Structurally normal N13 and N14 were absent; and N1, N6, N9, N12, N17, F and G chromosomes were single. A single copy each of the X and Y chromosome was detected. The presence of the Y chromosome was confirmed by fluorescent microscopy. |
| Derivation | The NCI-H441 cell line was derived by A.F. Gazdar, M. Brower and D. Carney and associates in 1982 from the pericardial fluid of a patient with papillary adenocarcinoma of the lung. |
| Clinical Data | male |
| Comments | Certified Reference Material produced under an ISO 17034 accredited process. Item has been tested for KRAS mutation (p.G12V c.35G>T) Electron microscopy shows multilamellar bodies and cytoplasmic structures resembling clara cell granules.
The cells can be cloned in soft agar with or without serum. The cell line expresses mRNA and protein of the major surfactant apoprotein (SP-A). Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Note: If desired, the serum can be reduced to 5%. |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 9,13 D5S818: 11,12 D7S820: 10 THO1: 9.3 TPOX: 8,10 vWA: 17 |
| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Population Doubling Time | 58 hrs in medium with serum; 99 to 138 hrs in serum-free medium |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| Year of Origin | 1982 |
| References | Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086 O'Reilly MA, et al. Differential effects of glucocorticoid on expression of surfactant proteins in a human lung adenocarcinoma cell line. Biochim. Biophys. Acta 970: 194-204, 1988. PubMed: 3382698 O'Reilly MA, et al. In vitro translation, post-translational processing and secretion of pulmonary surfactant protein B precursors. Biochim. Biophys. Acta 1011: 140-148, 1989. PubMed: 2713400 Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 Baatz JE, et al. Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture. Proc. Natl. Acad. Sci. USA 91: 2547-2551, 1994. PubMed: 8146151 Tamura T, Stadtman TC. A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity. Proc. Natl. Acad. Sci. USA 93: 1006-1011, 1996. PubMed: 8577704 Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
