Gene product: tyrosinase-related protein 1(homolog of b-locus protein, melanoma antigen gp 75) [CAS2]
Alleles: A2, A2, A1, A1, A2, A1
Insert Size (kb)
2.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; BglII--5.8; AvaI--5.8; HincII--3.4, 2.4; PstI/PvuII--1.6, 1.5, 1.0, 0.8, 0.4.
This clone, which was not derived from the same cell line as the published sequence (S7), does not have the HindIII site at nucleotide 748. This is probably due to polymorphism. It does have a BamHI site at 929.
Either an AvaI/NdeI or an SmaI/NdeI digest gives a 1.7 kb fragment containing the protein-coding region except for a putative signal peptide.
The vector lacks the SV40 promoter.
This is a full length cDNA clone; coding sequences extend from nucleotide 130 through 1710.
References
Cohen T, et al. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein. Nucleic Acids Res. 18: 2807-2808, 1990. PubMed: 2111010
