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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 3.9000000953674320

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752

Vector: pBSL14 (plasmid)

Construction: pUC18, pK18

Marker(s):ampR,kanR

Construct size (kb): 3.900000095367432

Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
MCS: HindIII...EcoRI
MCS: HindIII...NheI

contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 1.1; HindIII--2.8, 1.1; BamHI--2.7, 1.2.

The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites.

Plasmid with an easily purified kanR cassette (flanked by direct repeats of the pUC18 multiple cloning site) for use in construction.

Temperature: 37.0°C