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For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).

Construct size (kb): 3.63

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: KpnI
Vector end: SacI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:

Vector end (5'): KpnI

Vector end (3'): SacI

Excise insert: XhoI + SmaI

Promoter giving the sense strand: T3

Promoter giving the antisense strand: T7

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioC
Gene name: biotin synthesis protein
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: Modification: KpnI linker
Insert 3' end: Modification: SacI linker
Insert size (kb): 0.77
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-756 of the insert correspond to
nucleotides 4190-4945 of J04423.

Insert lengths(kb): 0.77

Gene product: biotin synthesis protein [bioC]

Insert end (5'): KpnI

Insert end (3'): SacI linker

For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).

The insert contains nt 4190-4945 of Genbank J04423.

The insert contains the following restriction sites (approximate kb from the 5' end): RsaI--0.14; SphI--0.48; SspI--0.65.

The following enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI.

Restriction digests of the clone give the following sizes (kb): SmaI--3.4; SmaI/XhoI--2.7, 0.78; XhoI--3.4.