| 產品名稱 |
hIPL clone #4 |
| 商品貨號 |
B187811 |
| Designations |
hIPL clone #4 |
| GenBank Number |
AF001294
|
| Species |
Homo sapiens, human |
| Depositors |
D Frank, B Tycko |
| Vector |
Construct size (kb): 3.727999925613403 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IPL
Gene name: imprinted in placenta and liver
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11p15.5
Strain: Human
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.76
Cross references: DNA Seq. Acc.: AF001294
Nucleotides 1-760 of the insert correspond to
nucleotides 1-760 of AF001294. Insert lengths(kb): 0.7599999904632568 Gene product: imprinted in placenta and liver [IPL] |
| Insert Size (kb) |
0.760 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII/SacI--2.8, 0.9; HindIII--3.7. Insert contains the following restriction sites (approximate kb form the 5' end): ClaI--0.35kb; PstI--0.38kb, 0.58kb; NotI--0.44kb; BglII--0.47kb; XmnI--0.59; BamHI--0.61kb; XhoII--0.61kb. |
| References |
Qian N, et al. The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis. Hum. Mol. Genet. 6: 2021-2029, 1997. PubMed: 9328465
|
