| 產品名稱 |
pCMV5FIX |
| 商品貨號 |
B187667 |
| Designations |
pCMV5FIX |
| GenBank Number |
J00136
|
| Species |
Homo sapiens, human |
| Applications |
in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector |
Construct size (kb): 7.400000095367432 |
| Insert |
DNA: cDNA Insert information: Insert size (kb): 2.8 Gene : F9, coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) DNA : cDNA Source : Human Genbank accession : J00136 Insert ends : BamHI, XbaI Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9] Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb) |
2.800 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--4.6, 2.9; BamHI--7.6; BglII--7.6; EcoRI--4.9, 2.7. Digestion with EcoRI produces fragments of 4.9 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Corresponds to the wild-type sequence. |
| References |
Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778
Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370
Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159
Wing-Fai Cheung, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
