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Distribution host: Escherichia coli DH10B

Size (kb): 4.6479997634887700

DESCRIPTION OF VECTOR:
Intact vector size: 4.648
Type of vector: plasmid
Cloning sites: PacI PmeI BglII
Polylinker sites:
Construction: pCRTOPO2.1
Features (with orientation and position when available):

Vector: pFa6a-KANMX6-pADH1 (plasmid)

Construction: pCRTOPO2.1

Marker(s):ampR

Construct size (kb): 4.64799976348877

cloning vector

Restriction digests of the clone give the following sizes (kb): BglII--4.6; SalI/BglII--3.9, 0.7; EcoRI--4.6.

The marker and promoter are flanked by the F4 primer site (5') and the R6 primer site (3'). The size of the F4/R6 PCR product should be 2005 nt.

F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.

Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.

Temperature: 37.0°C

component of:ATCC 87819