Restriction digests of the clone give the following sizes (kb): BglI--2.0, 1.8 (doublet); EcoRI--5.6; PstI--5.0, 0.4, 0.35; SalI--5.6; XbaI--4.3, 1.6. The insert contains the following restriction sites (nt from the 5' end): AccI--209; AvaI--1026; BglI--1207; NdeI--1212; PstI--329, 631; PvuII--1050, 1105; SalI--208. Corresponds to nt 5-1506 of the reported sequence and includes the complete coding region. Detects a transcript of 2.7 kb in rat pancreas and rat acinar carcinoma. |
