| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.8, 4.2, 1.8, 0.9, 0.8, 0.5, 0.4; PvuII--6.8, 2.3 (doublet), 1.2, 0.3; EcoRI--8.6, 5.0; BamHI--11.0, 2.5; HindIII--11.0, 2.5. The insert contains the following restriction sites (approximate kb from the 5' HindIII site): BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9; EcoRI--8.0. Distributed in aliquots of 1 ug (40 ng/ul). |
